How many seeds do you need to regenerate an accession maintaining its genetic integrity?

The proposed standard reads: A minimum of 100 plants (TO BE CHECKED) for cross-pollinating species and 50 (TO BE CHECKED) plants for self-pollinating species should be used for the regeneration in order to capture at least 95% of the genes/alleles.

 A paper by Lawrence (2002) is cited which says that 12 plants should be sufficient to reduce genetic drift to an acceptable level provided a biparental mating procedure is used to produce fresh seeds.

Another comments cites Crossa (1995) and suggests the following wording: A sample size of 130-200  individuals should be used for the regeneration  to capture the high probability of  genes/alleles.

What is the solution?


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