Regeneration
Regeneration
How many seeds do you need to regenerate an accession maintaining its genetic integrity?
The proposed standard reads: A minimum of 100 plants (TO BE CHECKED) for cross-pollinating species and 50 (TO BE CHECKED) plants for self-pollinating species should be used for the regeneration in order to capture at least 95% of the genes/alleles.
A paper by Lawrence (2002) is cited which says that 12 plants should be sufficient to reduce genetic drift to an acceptable level provided a biparental mating procedure is used to produce fresh seeds.
Another comments cites Crossa (1995) and suggests the following wording: A sample size of 130-200 individuals should be used for the regeneration to capture the high probability of genes/alleles.
What is the solution?
